Table 2.
Direct application of zVAD promotes hair cell survival in vivo after 3 d of streptomycin treatment
|
|
|
Streptomycin (1200 mg/kg) |
||||||
|---|---|---|---|---|---|---|---|---|
|
|
Saline Alone
|
Alone
|
Carrier
|
50 μm zVAD
|
100 μm zVAD
|
|||
| Extrastriolar | 154.8 ± 8.5 | 108.5 ± 5.4 | 79.7 ± 4.7 | 146.1 ± 12.6 | 147.4 ± 12.6 | |||
| (70.1 ± 3.5%) | (51.5 ± 3.0%) | (94.4 ± 8.1%) | (95.2 ± 8.1%) | |||||
| Striolar | 80.7 ± 5.1 | 36.6 ± 10.4 | 25.4 ± 2.2 | 51.8 ± 9.1 | 59.6 ± 7.5 | |||
|
|
|
(45.4 ± 12.9%)
|
(31.5 ± 2.7%)
|
(64.2 ± 11.3%)
|
(74.0 ± 9.3%)
|
|||
Chickens were implanted with an osmotic pump containing zVAD or carrier and received daily injections of streptomycin or saline for 3 d. One day after the last drug treatment, the animals were killed, and the utricles were removed from the ear containing the pump and processed for calretinin immunohistochemistry. Calretinin+ cells were quantified in 10,000 μm2 regions of both the extrastriolar (six regions per organ) and striolar (four regions per organ) areas. Mean values (±SEM) from four to eight organs are shown. The percentage of surviving hair cells (relative to controls) is presented below the hair cell densities.