Figure 9.

Forskolin does not reduce postsynaptic NMDA receptor function. A, Direct pressure application of NMDA (2 mm) induced voltage-sensitive current responses (puff response) in a spiny neuron. Note the reversal potential at approximately +20 mV. B, In the same cell shown in A, the current–voltage plot of the puff response showed voltage dependence similar to that of the late component of the EPSC evoked with electrical stimulation. C, Summary plot of the change in the puff response and the late component of the EPSC by forskolin application. Filled symbols represent two cells where puffs and electrical stimulation were interleaved, one of which is shown in D. D₁, Forskolin reduced the EPSC but not the puff response in a spiny neuron. Subsequent application of APV blocked both the late component of EPSC and the puff response. D₂, Example traces of the EPSC. D₃, Example traces of the puff response. Holding potential was +40 mV.