Figure 5.
Expression of APP isoforms. A, Northern blot analysis for APP, APLP1, and APLP2. Small numbers represent the days after being plated on polyO/L-coated dishes. B, Northern blot analysis for APP, APLP1, and APLP2 in neurons derived from wild-type or knock-in ES cells after 23 d of differentiation. C, RT-PCR analysis of the splicing at exon 15 in differentiating cells derived from wild-type or knock-in ES cells after 4, 9, and 23 d of differentiation. M represents markers in all figures. D, RT-PCR analysis of the expression of APP770, APP751, and APP695. PCR products were submitted to Southern blot analysis using a 273 bp fragment of APP. E, RT-PCR analysis of the expression of APP770, APP751, and APP695 in differentiating cells derived from wild-type or knock-in ES cells after 4, 9, and 23 d of differentiation. PCR products were submitted to Southern blot analysis using a 273 bp fragment of APP. Each of the results was reproduced at least three times.