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. 2003 Sep 17;23(24):8513–8525. doi: 10.1523/JNEUROSCI.23-24-08513.2003

Figure 8.

Figure 8.

Secretion of Aβ in the culture medium of ES-derived neurons. wtES and knock-in ES cells were similarly differentiated by our protocol, and the cultured media were collected every 3 d during the period between 7 and 28 d after being plated on polyO/L-coated dishes. The concentrations of Aβ40 (circles) and Aβ42 (triangles) secreted from wtES-derived neurons (closed symbols) or knock-in ES-derived neurons (open symbols) were determined by two-site ELISA (A). Values are means ± SE of six of seven independent series of similar experiments. The result from one series of the experiment, in which each obtained value deviated remarkably from the values of the other six experiments, was excluded because values remeasured in this experiment were not reproducible. Aβ42/total Aβ (Aβ40+Aβ42) ratios of wtES-derived neurons (closed circles) or knock-in ES-derived neurons (open circles) were calculated from the Aβ amounts (B). Statistical analysis was performed with one-way ANOVA, followed by a post hoc test, in which p < 0.05 was assessed as significant. Asterisks indicate a significant difference between wtES- and knock-in ES-derived neurons.