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. 2003 Oct 1;23(26):8872–8880. doi: 10.1523/JNEUROSCI.23-26-08872.2003

Figure 3.

Figure 3.

Okadaic acid decreases the binding of tau to microtubules in OLN-t40 cells. Cells were treated with OA or LiCl as indicated for 24 hr. Cell lysates were separated into cytoskeletal (P) and soluble (S) fractions after MT assembly and subjected to immunoblot analysis using polyclonal tau 17026 and MAb anti-α-tubulin antibodies. OA leads to the detachment of tau from the MTs, as indicated by an increase of tau in the soluble fraction and a decrease in the cytoskeletal fraction. LiCl increases the binding affinity of tau to MTs, as indicated by an increase of tau in the cytoskeletal fraction.