Table 1.
Concentrations of Aβ in the ISF, CSF, and tissue lysates in young and aged mice
|
|
Mean ± SEM concentration |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 months
|
12-15 months
|
||||||||||
| Tissue-fluid
|
Aβ1−x
|
Aβ40
|
Aβ42
|
Aβ1−x
|
Aβ40
|
Aβ42
|
|||||
| ISF-hippo (pg/ml) | 727.7 ± 92.1 | 224.4 ± 23.3 | 93.6 ± 16.9 | 626.4 ± 93.9 | 345.9 ± 52.2 | 73.9 ± 38.9 | |||||
| ISF-striatum (pg/ml) | 207.1 ± 31.8 | 142.1 ± 19.5 | |||||||||
| CSF (pg/ml) | 11337 ± 699.7 | 5058 ± 496.0 | 2375 ± 336.8 | 8451 ± 915.3 | 4272 ± 898.7 | 961 ± 170.0 | |||||
| Hippo-carbonate (pg/mg protein) | 255.0 ± 42.9 | 70.1 ± 14.5 | 80.3 ± 15.4 | 1147.6 ± 383.7 | 93.2 ± 29.7 | 692.5 ± 194.9 | |||||
| Hippo-guanidine (pg/mg protein)
|
1126 ± 135.9
|
105 ± 21.7
|
291 ± 30.4
|
87476 ± 29714
|
8159 ± 2775
|
66214 ± 22315
|
|||||
The concentration of ISF eAβ was assessed by microdialysis (n = 21). CSF was isolated from the cisterna magna and directly analyzed by ELISA (n = 6). Hippocampal tissues were processed by sequential extraction in carbonate buffer and then 5 m guanidine (n = 6). Tissue Aβ values are normalized to protein concentrations for each sample. Hippo, Hippocampus.