Table 1.
Properties and effects of various Aβ fragments
|
Aβ fragment |
Precipitates detected under light microscopy |
Structural properties analyzed by SDS-PAGE |
Intracellular actin aggregation |
Effects on axonal transport |
|---|---|---|---|---|
| Aβ15-20 amide | − | Low molecular weight forms (<1 kDa) | − | No effect |
| Aβ31-35 | − | Low molecular weight forms (<1 kDa) | + | Inhibitory |
| Aβ25-35 | + | Low molecular weight forms (~1 kDa) | + | Inhibitory |
| Aggregates | ||||
| Fresh Aβ1-40 | − | Monomers | − | No effect |
| Incubated Aβ1-40 | + | Monomers | − | No effect |
| Aggregates | ||||
| Fresh Aβ1-42 | − | Monomers | − | No effect |
| Pentamers | ||||
| Hexamers | ||||
| Incubated Aβ1-42 | − | Monomers | + | Inhibitory |
| Pentamers | ||||
| Hexamers | ||||
|
|
|
High molecular weight forms (30-100 kDa)
|
|
|
Aβ fragments dissolved in HEPES-buffered salt solution, pH 7.4, at a concentration of 20 μM were used for light microscopic analysis, intracellular actin observation with rhodamine-phalloidin, and axonal transport study under video-enhanced microscopy. Ten micrograms of each Aβ fragment dissolved in an aqueous solution were subjected to SDS-PAGE. +, Detected; −, not detected.