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. 2003 Oct 1;23(26):8949–8954. doi: 10.1523/JNEUROSCI.23-26-08949.2003

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Copyright © 2003 Society for Neuroscience 0270-6474/03/238949-06.00/0

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Figure 2. — PACAP activation of Rap1 and induction of calcium influx is impaired in Nf1-/- astrocytes. a, Serum-starved Nf1+/+ and Nf1-/- astrocytes stimulated with PACAP were analyzed for Rap1 activation. The top panel denotes active Rap1 (Rap1-GTP bound to GST-RalGDS), whereas bottom panel demonstrates the total Rap1 in each sample. Significantly reduced Rap1 activation is observed in the Nf1-/- compared with Nf1+/+ astrocytes. b, Serum-starved Nf1+/+ and Nf1-/- astrocytes stimulated with PACAP in presence of 45 Ca²⁺ demonstrate decreased calcium influx in Nf1-/- (black bar) compared with Nf1+/+ (white bar) astrocytes. Each error bar represents mean ± SD. Asterisks denote statistically significant differences (p = 0.001).