Figure 4.
Ectopic expression of NF1GRD partially rescues the impaired cAMP response to PACAP in Nf1-/- astrocytes. Whereas reduced cAMP levels were observed in untransduced and vector transduced Nf1-/- (black bars) compared with Nf1+/+ (white bars) astrocytes, a partial restoration of this response was observed in astrocytes expressing the NF1GRD fragment. No effect on cAMP generation was observed in Nf1+/+ astrocytes expressing the NF1GRD fragment. Expression of KT3-tagged NF1GRD in Nf1-/- MSCV-NF1GRD-Pac treated astrocytes is shown in the inset. Each error bar represents mean ± SD. Asterisks denote statistically significant differences (*p = 0.001; **p = 0.005).