Figure 1.
a, Measurement of human Aβ1-40 (□) and Aβ1-42 (▪) in cortex and hippocampus of PS2APP mice at ages 5, 9, 13, and 17 months. Soluble (top bar graphs) and insoluble (bottom bar graphs) peptides obtained after DEA and urea extractions, respectively, were quantified by ELISA. The mean and SEM values of 6-10 mice are represented. b, Western blot analysis of APP, 12 kDa β-cleaved fragment of APP (C-99), and Aβ (Abeta) in brain homogenate of individual PS2APP mice at ages 4, 6, 9, 11, 14, and 16 months and a 16 month nontransgenic control. The data show an age-related accumulation of Aβ, with some inter-animal variability; e.g., compare both lanes at age 14 months.