Figure 6.

On-section immunogold labeling after cryofixation using an anti-Aβ monoclonal antibody (BAP-2) visualized by a goat anti-mouse secondary antibody conjugated to 10 nm colloidal gold. Overviews and details (a-d) of electron micrographs from sections through 5 (c)- or 9 (a, b, d)-month-old transgenic mouse brains in which Aβ molecules have been labeled with anti-Aβ antibodies (BAP-2) bound to tiny colloidal gold spheres (each seen as a black dot). Immunoreactivity reveals the localization of Aβ. P, Plaque core; AF, fibrils of Aβ; M, microglia; DN, dystrophic neurites; NF, nonfibrillar Aβ. Scale bars: a, 10 μm; b, 200 nm; c, 250 nm; d, 500 nm.