Table 1.
Summary of studies examining PS2APP mice and age-matched controls in tests of neurological function
|
Age in months |
|
Body weight |
Body temperature |
Shock threshold |
Locomotor activity |
Grip strength |
|
One meter swim |
Rotarod |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
Flinch
|
Jump
|
LMA
|
Rears
|
Wire test
|
16 rpm
|
32 rpm
|
||||||||
| 4 | WT | 30.8 ± 0.5 | 38.5 ± 0.1 | ND | ND | 4950 ± 257 | 3020 ± 277 | 117 ± 4 | 26 (9-31) | 2.8 ± 0.1 | 120 (100-120) | 25 (20-66) | |||
| PS2APP | 27.9 ± 0.6* | 38.5 ± 0.1 | ND | ND | 7311 ± 584* | 2598 ± 211 | 73 ± 4* | 11 (6-17)* | 3.5 ± 0.2* | 120 (93-120) | 38 (26-57) | ||||
| 8 | WT | 40.3 ± 0.2 | 38.4 ± 0.1 | 0.13 ± 0.01 | 0.44 ± 0.03 | 3752 ± 367 | 1093 ± 157 | 121 ± 3 | 24 (7-41) | 2.7 ± 0.1 | 65 (45-104) | 21 (14-25) | |||
| PS2APP | 37.6 ± 0.8 | 38.4 ± 0.1 | 0.18 ± 0.01* | 0.61 ± 0.02* | 5495 ± 488* | 806 ± 77 | 73 ± 6* | 22 (12-37) | 3.7 ± 0.1* | 38 (28-71) | 15 (11-21) | ||||
| 12 | WT | 44.5 ± 1.3 | 38.6 ± 0.2 | 0.13 ± 0.01 | 0.60 ± 0.06 | 3005 ± 316 | 1971 ± 213 | 122 ± 4 | 10 (4-15) | 3.1 ± 0.1 | 35 (21-92) | 19 (14-27) | |||
| PS2APP | 42.9 ± 2.0 | 38.3 ± 0.2 | 0.19 ± 0.01* | 0.70 ± 0.06* | 4169 ± 343* | 1815 ± 145 | 107 ± 7 | 10 (4-18) | 3.9 ± 0.1* | 78 (16-104) | 11 (8-17) | ||||
| 16 | WT | 49.0 ± 1.9 | 38.0 ± 0.2 | 0.13 ± 0.01 | 0.38 ± 0.04 | 2542 ± 188 | 2246 ± 525 | 118 ± 6 | 9 (7-14) | 3.7 ± 0.2 | 20 (12-43) | 10 (7-13) | |||
|
|
PS2APP
|
36.9 ± 1.9*
|
37.7 ± 0.1
|
0.18 ± 0.04*
|
0.52 ± 0.03*
|
4395 ± 476*
|
2544 ± 422
|
89 ± 3*
|
3 (2-5)*
|
4.2 ± 0.2*
|
73 (68-81)*
|
31 (17-54)*
|
|||
n = 13-18 per group. Locomotor activity (LMA)/rearing: Mice were placed in a novel activity chamber for 60 min. Wire maneuver: Mice were placed by forepaws on an elevated wire rod and latency to fall is noted. Cut-off time was 60 sec; best score from three attempts is recorded. Grip strength: Mice were forced to pull on a strain gauge; release point is recorded. Best score from five attempts is shown. One meter swim test: Mice were placed in a straight swim tank (1 m long × 6 cm wide). Latency to swim distance and climb onto platform is noted. Mice were given three trials × 3 d; fastest time is recorded. Rotarod: Mice were placed on a constant speed rotorod; latency to fall noted. Cut-off time was 120 sec. Best score from three trials is taken. Two speeds were used: 16 rev/min and 32 rev/min. In addition, rectal body temperature, coat appearance, secretory signs, and body posture were also noted. Data are expressed as means ± SEM or median and interquartile range (wire and rotorod test).
p < 0.05 versus wild-type (WT) mice at equivalent age.