Figure 3.
Negative labeling of motoneurons for GLYT2 mRNA and GAD67 mRNA. A vagal motoneuron labeled with Neurobiotin (a1, arrow) exhibits ChAT immunoreactivity (a3, arrow) but is GLYT2-negative (a2). a1, a2, and a3 are the same area. This neuron was activated antidromically by electrical stimulation (arrow) of the vagus nerve (a4). Intracellular activity (a5, top trace) exhibited decrementing expiratory activity. a5, Bottom trace, Integrated phrenic nerve activity. Another vagal motoneuron labeled with Neurobiotin (b1, arrow) is GAD67-negative (b2). b1 and b2 are the same area. Scale bars: 50 μm for photomicrographs. Calibration: a4, 5 msec, 20 mV; a5, 1 sec, 20 mV.