Figure 2.

Duration of the IL-1β effect on NMDA-induced [Ca²⁺]_i increase. A, Trace 1 (gray) depicts a representative Ca²⁺ trace showing the response of hippocampal neurons to four consecutive applications of 10 μm NMDA, the first in the presence (6 min preincubation) and the following in the absence of 0.05 ng/ml IL-1β. Trace 2 (black) depicts a representative Ca²⁺ trace showing the response of hippocampal neurons to four consecutive applications of 10 μm NMDA done in the absence of IL-1β. Intracellular Ca²⁺ increase was determined by loading hippocampal neurons with fura-2 AM and measuring fura-2 fluorescence ratio signal in a double-wavelength fluorimeter. B, Average peak Ca²⁺ response to four consecutive applications of NMDA as reported in A. Values are the mean ± SE of 13 independent samples. ^**p < 0.01 and ^*p < 0.05 versus control; ANOVA followed by Tukey's test.