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. 2003 Sep 24;23(25):8722–8732. doi: 10.1523/JNEUROSCI.23-25-08722.2003

Figure 2.

Figure 2.

The effects of BDNF on transmitter-induced currents. A, Example of IGABA recorded from GABAergic (top) and glutamatergic (middle) cells and Iglu recorded from glutamatergic cells (bottom). IGABA were recorded using gramicidin D, and Iglu were recorded using amphotericin B. BDNF was added at t = 0. B, Summary of the normalized amplitude of IGABA and Iglu at various times before and after BDNF treatment (black bar). At GABAergic cells, BDNF caused an increase in IGABA amplitude (164 ± 13% of control), but at glutamatergic cells, BDNF caused no significant change in IGABA or Iglu amplitude (94 ± 10 and 92 ± 6%, respectively). No significant difference was found between IGABA recorded with gramicidin D and amphotericin B, and the data were pooled. C, Summary of the average BDNF effect on transmitter-induced currents. Modulation factor defined as the ratio between the mean transmitter-induced current amplitude 10-20 min after BDNF application and that during the last 10 min of the control period. BDNF caused an increase in IGABA recorded from GABAergic cells (1.54 ± 0.15; p < 0.001; n = 10), but this effect was blocked in cells that had been preincubated with a TrkB antibody (20 μg/ml; 1.09 ± 0.08; n = 5; p > 0.1). Preincubation with rabbit IgG (50 μg/ml) did not block the BDNF effect (1.96 ± 0.43; n = 4; p < 0.05). Glu, Glutamate; ab, antibody.