Figure 2.
Antibody against Aplysia p38 kinase. A, An affinity-purified antibody raised against a peptide (PEAEKYDQSFEEMELG) from the Aplysia kinase specifically recognized a single component of approximately Mr 38 kDa, the size expected of p38 kinase, from homogenates of Aplysia pleural ganglia. Preincubation of the antibody with the peptide used as immunogen blocked the signal. B, Recombinant Ap-p38 kinase activated by mammalian MKK6 was incubated with human ATF2 and ATP, with or without Ap-p38 antibody. Kinase activity, monitored by the phosphorylation of ATF2 by Western blot with anti-phospho-ATF2 antibody, was completely blocked by the Ap-p38 antibody.