Figure 2.

A, Schematic illustrations of chimeric G16/gust proteins with different lengths of C terminal amino acids found in gustducin and their abilities to couple to T2Rs. +, Specifically responded to the ligand in dose-dependent manner; -, did not exhibit any responses. B, Immunoblot analysis of FLAG-tagged chimeric G16/gust subunits expressed in HEK293T cells, stained with the anti-FLAG M2 monoclonal antibody. The FLAG epitope tag did not influence the functional activity of G16/gust chimeras.