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. 2003 Aug 13;23(19):7246–7254. doi: 10.1523/JNEUROSCI.23-19-07246.2003

Figure 2.

Figure 2.

Immunocytochemical staining of neo+ cells and TUNEL studies of the PrV in wild-type and Drg11-/- mice. A, B, Neo staining (red) and Hoechst countstaining (green) of the PrV in wild-type (A,C) and Drg11 (B,D) mutants at E14.5. Arrowheads indicate the region where few Drg11-/-/neo+ cells were found. C,D, Higher magnification of A,B. E, Comparison of the number of Drg11+/- and Drg11-/- cells in the PrV at E14.5. Neo+ cells in Drg11+/- embryos at E14.5, 143.7 ± 8.1; in Drg11-/- embryos, 135.4 ± 5.2; p = 0.061 (p > 0.05). F,G, Neo staining (red) in the PrV of the wild-type embryos (F) and the mutant (G) at E16.5, Note that in the most ventral aspect (arrowheads) of the mutant PrV, Drg11-/- cells appear to avoid certain regions. H, Comparison of the number of Drg11+/- and Drg11-/- cells in the PrV at E14.5. Neo+ cells in Drg11+/- embryos at E16.5, 153.6 ± 8.7; in Drg11-/- embryos, 160.3 ± 13.7; p = 0.72 (p > 0.05). I,J, At E18.5, TUNEL studies revealed increased cell death in the mutant PrV (J, arrow) compared with the wild-type PrV (I, arrows). K, Comparison of the number of apoptotic cells in the PrV between wild-type and Drg11 mutant embryos at E18.5. The average number of apoptotic cells in the PrV. Wild-type embryo, 5.3 ± 1.4; Drg11 mutant, 15.0 ± 2.3; p = 0.015 (p < 0.05). Scale bars, 100 μm.