Figure 5.
Wortmannin sensitizes neurons to Aβ1-42 toxicity. A, TUNEL-positive neuronal cell death in neurons pretreated 1 hr with 200 nm or 10 μm wortmannin and exposed to 100 nm extracellular Aβ1-42 in the continued presence of wortmannin for 24 hr. Data represent the mean and SEM of three independent experiments. One-way ANOVA followed by Scheffé's compared wortmannin-treated cells with untreated cells; *p < 0.001. B, Western blot analysis of total PI3K in neurons treated with Aβ1-42 and wortmannin. C, Autoradiogram of phosphorylated phosphatidylinositol (PIP) from immunoprecipitated PI3K. The concentration of wortmannin was 200 nm. Wtm, Wortmannin; Ctl, control; ORI, origin.