Figure 1.

Morphology and physiology of immature retinal ganglion cells. A, Two cells filled with Alexa fluor 488 (left) and 568 (right). Cells at this age are not yet fully differentiated. Both cells are from a P3 animal. Scale bar, 50 μm. B, Examples of spontaneous activity recorded from four cells. Bursts of action potentials alternate with periods of quiescence. Resting potentials (V_m), top to bottom, -69, -71, -65, and -68 mV. Calibration: x, 1 min; y, 40 mV. The bursts of activity are superimposed on membrane depolarizations. One burst in the first trace is expanded in the inset to demonstrate the fluctuation in membrane potential. The inset includes a 3 sec trace.