Figure 2.

Confocal images showing the laminar location of PKCγ immunostaining in the dorsal horn of a rat with intact sciatic nerves. In a the dorsal horn has been scanned with transmitted light through a dark-field condenser, whereas PKCγ immunoreactivity is seen in b and the two images are merged in c. In the dark-field scan, lamina II appears dark because of the lack of myelin, and its ventral border (arrows) is clearly visible. The ventral edge of the band of PKCγ immunostaining corresponds closely to the lamina II-III border defined with dark-field illumination. The dorsal limit of this band was used to distinguish inner and outer halves of lamina II (IIi and IIo). Scale bar, 200 μm.