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Substance P causes a marked increase in the frequency of respiratory rhythm generated by E17 in vitro preparations. A, Rectified and integrated suction electrode recordings made from the pre-BötC of an E17 medullary slice preparation. B, Rectified and integrated suction electrode recordings of diaphragm EMG activity from an E17 brainstem-spinal cord preparation. Substance P was added to the perfusate during the period demarcated by the horizontal lines.
