Figure 6.

E₂ attenuation of the GABA_B response involves protein kinase Cδ. A-D, Representative traces of the baclofen responses in the presence of PKC inhibitors. Experiments were conducted as described in Figure 1. For the BIS or fast Ca²⁺ chelator BAPTA, cells were dialyzed for 15 min before baclofen application with BAPTA (replacing the 11 mm EGTA with 10 mm BAPTA) or BIS (100 nm), which were included in the patch pipette solution. E, Bar graph summarizing the effects of PKC inhibitors. The broad PKC inhibitor BIS and a selective PKCδ inhibitor rottlerin (5 μm) could reverse, but the selective inhibitor of conventional PKC isoforms Gö6976 (2 μm) or BAPTA could not block the attenuation of the baclofen response by E₂. BIS could not block the attenuation of the baclofen response by forskolin (FSK; 10 μm). Error bars represent the mean ± SEM of 4-11 cells tested per group. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.005 versus vehicle control.