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. 2003 Oct 22;23(29):9675–9686. doi: 10.1523/JNEUROSCI.23-29-09675.2003

Figure 2.

Figure 2.

Fn14 mRNA expression is induced in DRG neurons by axotomy. A, B, Northern blot analysis of Fn14 mRNA expression in uninjured E15, uninjured P3, adult axotomized (Axo), and adult contralateral (Con) L4-6 mouse DRGs. Samples were collected at 7 d (A) or at various times (B) after sciatic nerve injury. The ethidium bromide staining pattern of the 18S ribosomal RNA is shown to demonstrate the quantity of RNA loaded. The migration of 28S and 18S rRNA is shown at left, and the relative signal intensity is reported at the bottom of each lane. C, In situ hybridization shows colocalization of Fn14 mRNA in the fluoro-gold-labeled axotomized DRG (arrowheads) but not in control contralateral DRG sections. Scale bar: four left panels, 250 μm; two right panels, 50 μm. D, Northern blot analysis of TWEAK mRNA expression in uninjured E15, uninjured P3, adult axotomized, and adult contralateral L4-6 mouse DRGs and in proximal or distal injured nerve stumps. Samples were collected 7 d after sciatic nerve transection. The ethidium bromide staining pattern of the 18S ribosomal RNA is shown to demonstrate the quantity of RNA loaded.