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. 2003 Sep 3;23(22):8077–8091. doi: 10.1523/JNEUROSCI.23-22-08077.2003

Figure 1.

Figure 1.

Immunopurification of MiRP2-Kv2.1 and MiRP2-Kv3.1 complexes from mammalian brain. A, Northern blot analysis showing distribution of MiRP2 mRNA in adult human brain and spinal cord. Numbers indicate migration distances of 1.35, 2.4, and 4.4 kb markers. B, RT-PCR of MiRP2 from mRNA isolated from E18, 12 DIV rat primary hippocampal neurons. Numbers indicate corresponding molecular weight marker migration distances in base pairs. Products were amplified by PCR from water control, hippocampal neuron mRNA before RT-PCR, or hippocampal neuron mRNA after RT-PCR. The identity of the 596 bp amplicon was confirmed by sequencing as MiRP2 (M2). C, Anti-MiRP2 antibody immunoblots of rat MiRP2-transfected CHO cell lysate (rMiRP2 CHO), mock-transfected CHO cell lysate (con CHO), or rat brain membranes (rat brain m). D, Anti-Kv2.1 antibody immunoblots of crude rat brain membranes (rat brain m), Kv2.1-transfected CHO cell lysate (Kv2.1 CHO), or immunoprecipitations from rat brain membranes using antibodies raised against Kv2.1 (α-Kv2.1 IP), Kv3.1 (α-Kv3.1 IP), parvalbumin (α-parv IP), or MiRP-2 (α-MiRP2 IP). E, Anti-Kv3.1 antibody immunoblots of crude rat brain membranes (rat brain m), Kv2.1-transfected CHO cell lysate (Kv2.1 CHO), or immunoprecipitations from rat brain membranes using antibodies raised against Kv2.1 (α-Kv2.1 IP), Kv3.1 (α-Kv3.1 IP), parvalbumin (α-parv IP), or MiRP-2 (α-MiRP2 IP).