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. 2003 Sep 3;23(22):8077–8091. doi: 10.1523/JNEUROSCI.23-22-08077.2003

Figure 2.

Figure 2.

MiRP2 colocalizes with heterologously expressed Kv2.1 and Kv3.1 subunits in CHO cell plasma membranes. Fluorescence microscopy of fixed and permeabilized CHO cells, either nontransfected or transiently transfected with plasmids encoding HA-tagged MiRP2 or untagged Kv2.1 or Kv3.1. Cells were stained with monoclonal anti-HA and polyclonal anti-Kv2.1 or polyclonal anti-Kv3.1 primary antibodies, and Alexa Fluor 594-labeled (red) goat anti-rabbit IgG and FITC-labeled (green) goat anti-mouse IgG secondary antibodies. Scale bars, 10 μm. A, Red-green overlay of nontransfected cells stained with anti-HA and anti-Kv2.1 antibodies showing no signal. B, Red-green overlay of nontransfected cells stained with anti-HA and anti-Kv3.1 antibodies showing no signal. C, Red-green overlay of HA-MiRP2-transfected cell stained with anti-HA antibody showing predominantly cytoplasmic distribution of HA-MiRP2 (green) when transfected alone. D-F, HA-MiRP2 and Kv2.1 cotransfected cell stained with anti-HA and anti-Kv2.1 antibodies; D, green signal showing membrane distribution of HA-MiRP2; E, red signal showing membrane distribution of Kv2.1; F, red-green overlay showing colocalization (yellow) of HA-MiRP2 and Kv2.1. G-I, HA-MiRP2 and Kv3.1 cotransfected cell stained with anti-HA and anti-Kv3.1 antibodies; G, green signal showing membrane distribution of HA-MiRP2; H, red signal showing membrane distribution of Kv3.1; I, red-green overlay showing colocalization (yellow) of HA-MiRP2 and Kv3.1.