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. 2003 Sep 3;23(22):8077–8091. doi: 10.1523/JNEUROSCI.23-22-08077.2003

Figure 5.

Figure 5.

MiRP2 colocalizes with native Kv2.1 and Kv3.1 subunits in PC12 cell plasma membranes. Fluorescence microscopy of fixed and permeabilized PC12 cells, either nontransfected or transiently transfected with a plasmid encoding HA-tagged MiRP2. Cells were stained with monoclonal anti-HA, polyclonal anti-Kv2.1, or polyclonal anti-Kv3.1 primary antibodies, Alexa Fluor 594-labeled (red) goat anti-rabbit IgG and FITC-labeled (green) goat anti-mouse IgG secondary antibodies. Scale bars, 10 μm. A, Nontransfected PC12 cells stained with anti-HA and anti-Kv2.1 antibodies showing: left, green channel, no HA staining; center, red channel, punctate native Kv2.1 staining; right, red-green overlay. B, HA-MiRP2-transfected PC12 cells stained with anti-HA and anti-Kv2.1 antibodies showing: left, green channel, punctate HA-MiRP2 staining; center, red channel, punctate native Kv2.1 staining; right, red-green overlay showing HA-MiRP2/native Kv2.1 colocalization (yellow). C, Nontransfected PC12 cells stained with anti-HA and anti-Kv3.1 antibodies showing: left, green channel, no HA staining; center, red channel, punctate native Kv3.1 staining; right, red-green overlay. D, HA-MiRP2-transfected PC12 cells stained with anti-HA and anti-Kv3.1 antibodies showing: left, green channel, HA-MiRP2 staining; center, red channel, native Kv3.1 staining; right, red-green overlay showing HA-MiRP2/native Kv3.1 colocalization (yellow).