MiRP2 colocalizes with native Kv2.1 and Kv3.1 subunits in PC12 cell plasma membranes. Fluorescence microscopy of fixed and permeabilized PC12 cells, either nontransfected or transiently transfected with a plasmid encoding HA-tagged MiRP2. Cells were stained with monoclonal anti-HA, polyclonal anti-Kv2.1, or polyclonal anti-Kv3.1 primary antibodies, Alexa Fluor 594-labeled (red) goat anti-rabbit IgG and FITC-labeled (green) goat anti-mouse IgG secondary antibodies. Scale bars, 10 μm. A, Nontransfected PC12 cells stained with anti-HA and anti-Kv2.1 antibodies showing: left, green channel, no HA staining; center, red channel, punctate native Kv2.1 staining; right, red-green overlay. B, HA-MiRP2-transfected PC12 cells stained with anti-HA and anti-Kv2.1 antibodies showing: left, green channel, punctate HA-MiRP2 staining; center, red channel, punctate native Kv2.1 staining; right, red-green overlay showing HA-MiRP2/native Kv2.1 colocalization (yellow). C, Nontransfected PC12 cells stained with anti-HA and anti-Kv3.1 antibodies showing: left, green channel, no HA staining; center, red channel, punctate native Kv3.1 staining; right, red-green overlay. D, HA-MiRP2-transfected PC12 cells stained with anti-HA and anti-Kv3.1 antibodies showing: left, green channel, HA-MiRP2 staining; center, red channel, native Kv3.1 staining; right, red-green overlay showing HA-MiRP2/native Kv3.1 colocalization (yellow).