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. 2003 Sep 3;23(22):7967–7973. doi: 10.1523/JNEUROSCI.23-22-07967.2003

Figure 4.

Figure 4.

Preconditioning produces caspase-mediated cleavage of PARP-1. A, Western blots with antibody to the 89 kDa PARP-1 fragment were prepared from cultures 24 hr after sham or 15 min PC. The increase produced by preconditioning was attenuated in cultures treated with the caspase inhibitor DEVD-CHO (25 μm). Values above the bands denote the increase ± SE relative to the paired control (**p < 0.01; n = 3). B, Immunostaining for the 89 kDa PARP-1 cleavage product showed it to accumulate in neurons after preconditioning. The accumulation was almost totally blocked by 25 μm DEVD-CHO, as observed in the Western blots. Similar results were observed in three independent experiments. Scale bar, 100 μm.