Figure 1.

Tenascin-R mRNA and protein are expressed in zebrafish. A, Northern blot analysis of total RNA isolated from adult zebrafish brains yielded a band at 11 kb (lane 1). A probe to tenascin-C labeled a band at 7 kb (lane 2). Lengths of transcripts are indicated on the left; positions of molecular markers are indicated on the right. B, Western blot analysis using anti-EGF-L antibody on detergent lysates of heads of 3-d-old larvae, separated on the same gel, indicate reduction of immunoreactivity by morpholino1 and morpholino2 but not by control morpholino. Reprobing of the blot with an antibody to tubulin is depicted in the bottom row. Molecular weights of tenascin-R and tubulin are indicated on the right.