Table 1.
Comparison of training and probe trial performance of CREB mutant mice in the water maze
|
|
All controls n = 38 one-way ANOVA Linea |
All mutants n = 49 one-way ANOVA Lineb |
Mutants excl CamKCre7 n = 37 one-way ANOVA Line |
All animals n = 87 two-way ANOVA Genotypec |
Genotype × selectivityd |
All controls n = 38 mean ± SE |
αΔ n = 10 mean ± SE |
comp n = 13 mean ± SE |
NesCre n = 14 mean ± SE |
CamKCre7 n = 12 mean ± SE |
|---|---|---|---|---|---|---|---|---|---|---|
| Training | ||||||||||
| Escape latencye (sec) | ns | ns | ns | p<0.0007 | ns | 22.77 ± 1.07 | 27.17 ± 4.28 | 32.08 ± 2.85 | 31.06 ± 2.66 | 33.87 ± 3.91 |
| Path length (m) | ns | ns | ns | p<0.0031 | ns | 5.00 ± 0.22 | 6.52 ± 0.93 | 7.60 ± 0.57 | 6.44 ± 0.51 | 6.38 ± 0.83 |
| Search error (m × sec) | ns | ns | ns | p<0.0008 | ns | 12.09 ± 0.70 | 17.04 ± 3.31 | 20.90 ± 2.37 | 17.99 ± 2.13 | 19.48 ± 3.16 |
| Wall hugging (%) | ns | ns | ns | p<0.0008 | ns | 8.52 ± 0.71 | 20.90 ± 6.46 | 28.14 ± 5.76 | 17.63 ± 3.71 | 18.19 ± 4.24 |
| Probe trial 1 | ||||||||||
| Percentage of time in trained quadrant | ns | p<0.0656 | ns | ns | p<0.0234 | 35.78 ± 2.03 | 28.5 ± 2.11 | 26.39 ± 2.00 | 31.11 ± 3.70 | 40.87 ± 6.49 |
| Percent time in trained zone | ns | p<0.0226 | ns | ns | p<0.0121 | 24.58 ± 1.53 | 14.40 ± 2.24 | 14.49 ± 1.81 | 18.06 ± 3.53 | 28.12 ± 5.02 |
| Crossing preference (x/m) | ns | p<0.0149 | ns | ns | p<0.0016 | 0.19 ± 0.06 | -0.01 ± 0.07 | 0.05 ± 0.05 | 0.16 ± 0.09 | 0.44 ± 0.16 |
| Proximity (m) | ns | p<0.0346 | ns | ns | p<0.0364 | 0.49 ± 0.01 | 0.59 ± 0.02 | 0.59 ± 0.03 | 0.57 ± 0.04 | 0.46 ± 0.04 |
| Polar error (°) | ns | p<0.0368 | ns | ns | p<0.0613 | 77.10 ± 2.71 | 84.03 ± 2.18 | 88.87 ± 2.95 | 84.06 ± 5.11 | 69.06 ± 7.36 |
| Probe trial 2 | ||||||||||
| Percent time in trained quadrant | ns | p<0.0628 | ns | ns | p<0.0077 | 38.88 ± 2.49 | 32.83 ± 4.09 | 25.97 ± 2.25 | 34.78 ± 3.21 | 40.99 ± 5.56 |
| Percent time in trained zone | ns | p<0.0502 | ns | ns | p<0.0101 | 26.26 ± 1.97 | 19.13 ± 4.02 | 14.26 ± 1.94 | 21.88 ± 3.26 | 27.90 ± 4.33 |
| Crossing preference (x/m) | ns | ns | ns | ns | ns | 0.22 ± 0.07 | 0.24 ± 0.11 | 0.10 ± 0.06 | 0.14 ± 0.11 | 0.41 ± 0.16 |
| Proximity (m) | ns | ns | ns | ns | p<0.0432 | 0.48 ± 0.02 | 0.56 ± 0.05 | 0.58 ± 0.03 | 0.51 ± 0.03 | 0.47 ± 0.04 |
| Polar error (°)
|
ns
|
p<0.0408
|
ns
|
ns
|
p<0.0197
|
73.19 ± 3.13
|
83.33 ± 5.66
|
88.43 ± 3.29
|
77.52 ± 3.78
|
69.6 ± 6.04
|
ns, Not significant
a
αΔ + comp versus CamKCre7 versus NesCre
b
αΔ versus comp versus CamKCre7 versus NesCre
c
CREB deficient versus wild type
d
Forebrain selective (CamKCre7) versus whole NS (αΔ, comp, NesCre)
e
Escape latency and other training measures averaged over all acquisition trials