Figure 1.

Glia enhanced dopaminergic neurodegeneration induced by rotenone. A, Primary neuron—glia cultures were treated for 3 or 7 d with vehicle or 0.5—15 nm rotenone, and the capability of cultures to uptake DA was evaluated. B, Neuron—glia cultures were treated for 7 d with vehicle or 10 nm rotenone, and the selectivity of rotenone-induced neurodegeneration was determined by quantification of TH-IR and NeuN-IR neurons after immunocytochemical staining. C, Neuron—glia (N/G; •) or neuron-enriched (N; ▪) cultures were treated for 7 d with vehicle or 2.5—10 nm rotenone. [³H]DA uptake was measured to assess neurotoxicity to dopaminergic neurons. The results for DA uptake (A, C) and cell counts (B) are expressed as a percentage of the control cultures and are means ± SEM of three experiments performed in triplicate. ^*p < 0.05, ^**p < 0.005 compared with the corresponding control (A, B). ^*p < 0.05 compared with the corresponding rotenone-treated neuron-enriched cultures (C).