Figure 3.
Effect of the addition of microglia to neuron-enriched (N) cultures on rotenone-induced degeneration of dopaminergic neurons. Mesencephalic neuron-enriched cultures were supplemented with 5 × 104 microglia per well prepared from either gp91 phox+/+ or gp91 phox-/- mice. After 24 hr, the cultures were treated with vehicle or 5 nm rotenone, and [3H]DA uptake was determined 7 d after the treatment. Results are expressed as a percentage of the control cultures and are means ± SEM of three experiments performed in triplicate. p < 0.05 was considered statistically significant. All other comparisons were not significant.