Figure 4.

Rotenone stimulated release of superoxide in microglia from gp91 ^phox+/+ mice but not gp 91 ^phox-/- mice. Mouse primary microglia were seeded to 96 well plates. After 24 hr, microglia were pretreated with vehicle or 0.5 mm apocynin for 30 min. Afterward, microglia were stimulated with rotenone (5 or 10 nm) or corresponding vehicle. Superoxide production, measured as SOD-inhibitable cytochrome c reduction, was determined as described in Materials and Methods. There was no significant difference in the basal levels of superoxide production (control) between the cultures from gp91 ^phox-/- mice and those of their wild-type litter-mates. Results are a percentage of the control cultures and are expressed as means ± SEM of three experiments performed in triplicate. ^**p < 0.005 compared with the control. ⁺⁺p < 0.005 compared with corresponding rotenone-treated cultures.