Table 1.
Combination of antisera used in each double-immunolabeling reaction
|
Staining |
Primary antibodies |
Secondary antibodies |
|---|---|---|
| D5/ChAT | Rabbit anti-D5 | Alexa 598-conjugated chicken anti-rabbit (red) |
| Goat anti-ChAT | Alexa 488-conjugated chicken anti-goat (green) | |
| D5/PV | Rabbit anti-D5 | Alexa 598-conjugated goat anti-rabbit (red) |
|
|
Mouse anti-PV
|
CY2 goat anti-rat (green)
|
Alexa-conjugated secondary antibodies were supplied by Molecular Probes (Eugene, OR), and the cyanine-conjugated secondary antibody was supplied by Amersham Biosciences. ChAT, Choline acetyltransferase; PV, parvalbumin.