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. 2003 Oct 15;23(28):9403–9408. doi: 10.1523/JNEUROSCI.23-28-09403.2003

Figure 3.

Figure 3.

Forebrain ablation of NF-κB activity leads to increased cell death in organotypic hippocampal slice cultures of double transgenic mice. A, FeSO4-induced neuronal damage. Slice cultures from double transgenic tTA/hIκBα-AA or single transgenic hIκBα-AA mice were treated for 24 hr without (controls, top panels) or with 200 μm FeSO4 (bottom panels). B, PI fluorescence values measured 0 or 24 hr after FeSO4 treatment. C, Time course of kainate-induced neurodegeneration. Slice cultures from double transgenic tTA/hIκBα-AA or single transgenic hIκBα-AA mice were treated with 500 μm kainate for the indicated times. D, PI fluorescence values measured in untreated and kainate-treated slices at different time points. Neuronal damage was visualized by red PI fluorescence imaging. CA1, Hippocampal region CA1; CA3, hippocampal region CA3. E, Pseudocolor quantification of cell death 8 hr after KA treatment.