Table 2.

Changes in α - and β -globin and MPK-1 gene expression in sodium butyrate-treated R6/2 mice

		NaBu Txd R6/2 versus R6/2					Untreated R6/2 versus WT
mRNA	Region	Array	PCR	Northern blot	Array	PCR	Northern blot
MKP-1	Striatum	I	181%a		NC	75%
	Cortex	I		204%e	NC		81%
α-Globin	Striatum	I	206%c		NC	120%
	Cortex	I		159%c	NC		99%
β-Globin	Striatum	I	215%b		NC	104%
	Cortex	I		206%d	D		39%a
Preproenkephalin	Striatum	NC	101%	98%	D	52%b	62%a

Samples from sodium butyrate (NaBu)-treated R6/2, untreated R6/2, and wild-type (WT) mice (n = 4 from each group) were analyzed for differential mRNA expression by microarray, real-time PCR, and Northern blotting. Array data are expressed as follows: I, Increased; D, decreased; NC, no change (based on a minimum difference call cutoff of three of four independent comparisons using samples from individual mice; see Materials and Methods). PCR and Northern blot data are presented as mean values expressed as a percentage of the baseline group (either untreated R6/2 or wild-type mice) after normalization to β-actin signal (see Materials and Methods). ^aSignificantly different from comparison group (p < 0.05); ^bsignificantly different from comparison group (p < 0.02); ^csignificantly different from comparison group (p < 0.01); ^dsignificantly different from comparison group (p < 0.001); ^esignificantly different from comparison group (p < 0.0001).