Figure 5.

5-HT induces the translation of α-tubulin in isolated sensory neurites. Sensory cells were cultured for 5 d, at which time the cell bodies and 50-100 μm of the proximal axon segments were removed using a sharp microelectrode. The isolated sensory neurites were treated with five pulses of 5-HT (5HT) or with medium alone (mock), fixed, and immunostained with the monoclonal anti-α-tubulin antibody. 5-HT treatment produced an increase in α-tubulin immunoreactivity in the isolated sensory neurites. Note that there appear to be hotspots of α-tubulin immunoreactivity in the 5-HT-treated neurites. Scale bar: two left panels, 50 μm; two right panels, 12.5 μm.