EGFP expression in hypothalamic organotypic cultures after biolistic transfection with α-tubulin. EGFP (A, B), 3.5VPIII.EGFP.IGR2.1 (C, D), or OTIII.EGFP.IGR3.6 (E, F) constructs. See Materials and Methods for description of the EGFP constructs. Scale bars: A, C, E, 100μm; B, D, F, 50 μm.