Figure 3.

Effects of En paralog knock-out on the axonal arbor of neuron 6m. The panels show ventral views of whole-mount terminal ganglia containing antibody-intensified Lucifer Yellow fills of 6m, colored according to branch depth within the neuropil (white represents most ventral, green, most dorsal). The anterior of the ganglion is toward the top and the midline to the right of each panel. A, Typical control medial-type arborization. B, C, Arborizations after Pa-en2 dsRNA injection (En2-). The arbor in B appears similar to control, whereas that in C is lateral-type. D, E, Arborizations of neurons treated with Pa-en1 dsRNA (En1-). D illustrates the lateral-type appearance of most of the arbors, whereas E shows an unusual arbor with mixed characteristics and axon branches in both lateral and medial tracts. F, Typical 6m lateral-type arbor after application of both en dsRNAs.