Figure 7.

Effects of dsRNA dose on the axonal arbor of neuron 6m. The panels show ventral views of whole-mount terminal ganglia containing antibody-intensified Lucifer Yellow fills of 6m, colored according to branch depth within the neuropil (white represents most ventral, green, most dorsal). The anterior of the ganglion is toward the top and the midline to the right of each panel. A, Typical control medial-type arborization. B, C, Arborizations after treatment with a 1:200 dilution of an equimolar mixture of Pa-en1 and Pa-en2 dsRNAs. The arbor in B appears similar to control, whereas that in C is more similar to lateral-type. D,E, Arborizations of neurons treated with a 1:30 dilution of the dsRNA mix. D illustrates the lateral-type appearance of some of the arbors, whereas E shows an indeterminate arbor type. F, Typical 6m lateral-type arbor after an undiluted application of both en dsRNAs.