Figure 8.

Quantification of the effects of dsRNA dose on the axonal arbor of neuron 6m. A-C, Histograms of axon angle, anterolateral branches, and dorsal branches, comparing arbors from control 6m (n = 10) and 6d (n = 3) to those from neurons treated with a 1:200 dilution (n = 6) or 1:30 dilution (n = 7) of the mixed dsRNAs, and from neurons treated with the undiluted mixture (1, n = 9). Asterisks indicate significant differences (p < 0.05) from control values. Overall, there are no significant differences between the effects of the 1:200 dilution and Pa-en2 dsRNA alone (compare Fig. 4), or between the 1:30 dilution and Pa-en1 dsRNA alone. A, Axon angle is significantly reduced with a 1:30 dilution (p = 0.01), as in double knock-outs. The reduction with a 1:200 dilution was not significant (p = 0.07) because of the large variance. B, Anterolateral branches are significantly increased by both dsRNA dilutions (1:200: p = 0.03; 1:30: p = 0.0008). C, Dorsal branches are significantly decreased by both dsRNA dilutions (1:200: p = 0.001; 1:30: p < 0.0001). A 1:200 dilution of dsRNA is significantly less effective than the undiluted mixture (p = 0.007).