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. 2003 Aug 27;23(21):7863–7872. doi: 10.1523/JNEUROSCI.23-21-07863.2003

Figure 2.

Figure 2.

Colocalization of MC4R mRNA and CRH in the PVN. A, Microscopic images of dual in situ hybridization histochemistry of MC4R mRNA (35S-labeled riboprobe, clusters of green grains) and CRH mRNA (digoxigenin-labeled riboprobe, dark purple cells). Subdivisions of the PVN: dp, dorsal parvicellular; mpd, medial parvicellular, dorsal aspect; mpv, medial parvicellular, ventral aspect; pm, posterior magnocellular. B, High magnification of microscopic images showing double-labeled cells. Black arrows indicate cells double-labeled for MC4R mRNA and CRH mRNA. White arrows indicate cells labeled for MC4R mRNA only.