Figure 4.

Triple-labeling demonstrates apoE colocalization to CAA in vessels (arrows) in APPsw mice. Eighteen-month-old APPsw mice (A-H) and 18-month-old APPsw, Apoe-/- mice (I-L) were immunostained for apoE (A, E, I) and Aβ (B, F, J) and with the Congo red derivative X-34 as a marker of fibrillar amyloid (C, G, K). Although fibrillar Aβ (arrowheads) begins to deposit in the parenchyma of old APPsw, Apoe-/- mice at later ages, no evidence for CAA was found in these same mice. Scale bars: (shown in D for A-D; shown in L for E-L), 100 μm.