Table 2.

Ratio of Aβ 40:42 is increased in vessels from aged mice with CAA and is dependent on apoE

	Ratio of Aβ 40:42
	Whole brain	Parenchyma	Vessel
3 month APPsw	ND	2.65	1.48
18 month APPsw	ND	5.7	19.4
4 month PDAPP #1	0.27	0.28	0.19
4 month PDAPP #2	0.29	0.32	0.2
4 month PDAPP, E—/— #3	0.24	0.33	0.18
4 month PDAPP, E—/— #4	0.32	0.34	0.07
24 month PDAPP #5	0.3	0.3	6.1
24 month PDAPP #6	0.4	0.5	1.1
22 month PDAPP, E—/— #7	0.05	0.01	0.08
21 month PDAPP, E—/— #8	0.07	0.05	0.16

Aβ ELISA analysis of brain and vessel lysates confirms an elevated ratio of Aβ 40:42 in cerebral vessels of aged APPsw and PDAPP mice. Cerebral vessel lysates from aged PDAPP, Apoe—/— mice have an extremely low ratio of Aβ 40:42. The Aβ 40:42 ratio in young PDAPP, Apoe—/— mice is similar to that in young PDAPP mice. The levels of Aβ₄₀ and Aβ₄₂ in blood vessels could not be normalized to vessel protein because of the small amount of vessel tissue. In these young PDAPP and APPsw mice, ∼0.5-1% of brain Aβ is associated with vessels. In old mice, ∼1-5% of brain Aβ is associated with vessels in PDAPP mice and ∼10-30% in APPsw mice. ND, Not determined.