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. 2003 Jul 23;23(16):6586–6595. doi: 10.1523/JNEUROSCI.23-16-06586.2003

Figure 3.

Figure 3.

Two distinct types of dendritic filopodia based on drebrin staining. A, Double labeling of a typical dendrite at 14 DIV for drebrin and F-actin. Filopodium 1 shows diffuse staining of drebrin, and filopodium 3 shows the discontinuous staining of drebrin. However, drebrin clusters are observed at the middle region of filopodium 2 and at the basal region of filopodium 4. The drebrin clusters are overlapped with enriched F-actin area (arrows). Scale bar, 2 μm. B, Drebrin-immunofluorescent intensity along each filopodium in A. Each average intensity is indicated as a dotted line in each chart. A solid line in each chart indicates twice the average intensity of each filopodium. When a filopodium had a drebrin cluster whose maximum intensity exceeds the threshold of the solid line, it was defined as a cluster-type filopodium (right column). When a filopodium did not have such a drebrin cluster, it was classified as a diffuse-type filopodium (left column). AU, Arbitrary unit. C, Mean densities of diffuse-type and cluster-type filopodia at 7 DIV (n = 8 dendrites) and 14 DIV (n = 13 dendrites). The density of diffuse-type filopodia at 14 DIV is significantly lower than that at 7 DIV, whereas the density of cluster-type filopodia at 14 DIV is significantly higher than that at 7 DIV (*p < 0.01; **p < 0.0001; t test). Error bars indicate SEM. Histograms show means + SEM.