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Superficial-layer neurons project onto NR1-immunoreactive neurons in the deeper layers of the superior colliculus. A, BDA injection site in the sSC (the borders between the sSC and dSC are shown by the dashed line). B-F, Layer IV neurons that are strongly immunopositive for the NR1 subunit of the NMDA receptor (brown) are contacted on the soma or proximal dendrite by axons with numerous varicosities (black). Scale bars: A, 50 μm; (in B) B-F,20 μm.
