Figure 4.
PrPC labeling in neuronal cell bodies in the hippocampus of a wt FVB mouse. A, Pyramidal neuron. Gold particles were found in the Golgi complex (arrowheads) and were absent from coated rims (asterisks) of Golgi cisternae. B, Granule neuron from the dentate gyrus. Gold particles are present in the nuclear envelope (arrow), which is a part of the ER, on late endosomes-multivesicular bodies (mvb), and on the plasma membrane (arrowhead). C, Pyramidal neuron. Gold particles are found on the plasma membrane (arrowheads) and early endosomes (arrows) but not in clathrin-coated pits or clathrin-coated vesicles (asterisks). ee, Early endosome; er, endoplasmic reticulum; G, Golgi complex; m, mitochondria; mvb, late endosomal multivesicular body; n, nucleus; ne, nuclear envelope; pm, plasma membrane; asterisks, lumen of coated vesicles, pits, and rims. Scale bars, 200 nm.