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. 2003 Aug 6;23(18):6972–6981. doi: 10.1523/JNEUROSCI.23-18-06972.2003

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Copyright © 2003 Society for Neuroscience 0270-6474/03/236972-10.00/0

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Figure 8. — Cyclic changes in the hippocampal mossy fiber system during hibernation. A1-A4, Mossy fibers labeled by PSA-NCAM (arrowheads). Note the disappearance of staining during torpor and its progressive reappearance during arousal. B1-B5, Immunohistochemical reaction for synaptophysin in the stratum lucidum (arrowheads). Reactivity decreases and increases again with a similar time course as the PSA-NCAM labeling of mossy fibers. C1-C5, Double immunofluorescence for MAP2 (green) and AT8 (red), coexpression in yellow. Although MAP2 reactivity in the stratum lucidum (arrowheads) disappears in torpor and reappears during arousal with a similar time course as PSA-NCAM (A) and synaptophysin (B), an inverse pattern is observed for AT8 reactivity in corresponding pyramidal cell bodies. Scale bars: A, 300 μm; B, C, 50 μm.