Figure 5.

Immunoblotting with various antibodies conducted with whole-brain lysates. a, Lysates from 6-week-old mice were immunoblotted with antibodies against tau1/2 protein, the AT8 antibody against tau phosphorylated at Ser²⁰², and the AT180 antibody against tau phosphorylated at Thr²³¹. b, Lysates from 4.5- and 6-week-old mice were immunoblotted with AT8 antibody against tau phosphorylated at Ser²⁰². c, Lysates from 6-week-old mice were immunoblotted with antibodies against phospho-GSK3β (Ser⁹) and GSK3β protein. d, Lysates from 6-week-old mice were immunoblotted with antibodies against PP2A scaffold subunit (PR65) and PP2A catalytic subunits. wt, Wild type.