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. 2003 Sep 10;23(23):8318–8329. doi: 10.1523/JNEUROSCI.23-23-08318.2003

Figure 6.

Figure 6.

Photomicrographs showing P0-14 DIV slices double labeled with anti CaBP/L7 (A-C), with anti NeuN/CaBP (D, E), or with anti-parvalbumin-CaBP (F-H) antibodies. A-C, In a BrdU-treated slice, Purkinje cells are CaBP positive (A, green) and L7 positive (B, red), thus appearing in yellow (C). Note that all CaBP-positive cells are at least somewhat L7 positive (A-C, arrowheads). A control slice (D) and a BrdU-treated slice (E) double labeled with NeuN (red) and CaBP (green). Note that the number of NeuN-positive cells seems to be the same in treated and control slices. F-H, A BrdU-treated slice double labeled with parvalbumin (F, green) and CaBP (F, G). Note that some Purkinje cells are only positive for CaBP (arrowheads), whereas some are positive for both CaBP and parvalbumin (F-H-, stars). Stellate and basket cells are positive only for parvalbumin (F-H, arrows). Scale bar: A-C, 50 μm; D, E, 85 μm; F-H, 20 μm.